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Since their introduction to North America in the 1980s, research to develop effective control tools for invasive mussels (Dreissena polymorpha and D. rostriformis bugensis) has been ongoing across various research institutions using a range of testing methods. Inconsistencies in experimental methods and reporting present challenges for comparing data, repeating experiments, and applying results. The Invasive Mussel Collaborative established the Toxicity Testing Work Group (TTWG) in 2019 to identify “best practices” and guide development of a standard framework for dreissenid mussel toxicity testing protocols. We reviewed the literature related to laboratory-based dreissenid mussel toxicity tests and determined the degree to which standard guidelines have been used and their applicability to dreissenid mussel testing. We extracted detailed methodology from 99 studies from the peer-reviewed and gray literature and conducted a separate analysis for studies using presettlement and postsettlement mussels. We identified specific components of methods and approaches that could be refined or standardized for dreissenid mussels. These components included species identification, collection methods, size/age class distinction, maintenance practices, testing criteria, sample size, response measures, reporting parameters, exposure methods, and mortality criteria. We consulted experts in the field of aquatic toxicology and dreissenid mussel biology on our proposed. The final recommendations contained in the present review are based on published standard guidelines, methods reported in the published and gray literature, and the expertise of TTWG members and an external panel. In addition, our review identifies research needs for dreissenid mussel testing including improved methods for early–life stage testing, comparative data on life stages and between dreissenid mussel species, inclusion of a reference toxicant, and additional testing of nontarget species (i.e., other aquatic organisms). Environ Toxicol Chem 2023;00:1–18. © 2023 His Majesty the King in Right of Canada. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC. Reproduced with the permission of the Minister of Environment and Climate Change Canada. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA. 

Hydractinia is a colonial marine hydroid that exhibits remarkable biological properties, including the capacity to regenerate its entire body throughout its lifetime, a process made possible by its adult migratory stem cells, known as i-cells. Here, we provide an in-depth characterization of the genomic structure and gene content of two Hydractinia species, H. symbiolongicarpus and H. echinata, placing them in a comparative evolutionary framework with other cnidarian genomes. We also generated and annotated a single-cell transcriptomic atlas for adult male H. symbiolongicarpus and identified cell type markers for all major cell types, including key i-cell markers. Orthology analyses based on the markers revealed that Hydractinia's i-cells are highly enriched in genes that are widely shared amongst animals, a striking finding given that Hydractinia has a higher proportion of phylum-specific genes than any of the other 41 animals in our orthology analysis. These results indicate that Hydractinia's stem cells and early progenitor cells may use a toolkit shared with all animals, making it a promising model organism for future exploration of stem cell biology and regenerative medicine. The genomic and transcriptomic resources for Hydractinia presented here will enable further studies of their regenerative capacity, colonial morphology, and ability to distinguish self from non-self. 

Connected vehicle (CV) technology brings both opportunities and challenges to the traffic signal control (TSC) system. While safety and mobility performance could be greatly improved by adopting CV technologies, the connectivity between vehicles and transportation infrastructure may increase the risks of cyber threats. In the past few years, studies related to cybersecurity on the TSC systems were conducted. However, there still lacks a systematic investigation that provides a comprehensive analysis framework. In this study, our aim is to fill the research gap by proposing a comprehensive analysis framework for the cybersecurity problem of the TSC in the CV environment. With potential threats towards the major components of the system and their corresponding impacts on safety and efficiency analyzed, data spoofing attack is considered the most plausible and realistic attack approach. Based on this finding, different attack strategies and defense solutions are discussed. A case study is presented to show the impact of the data spoofing attacks towards a selected CV based TSC system and corresponding mitigation countermeasures. This case study is conducted on a hybrid security testing platform, with virtual traffic and a real V2X communication network. To the best of our knowledge, this is the first study to present a comprehensive analysis framework to the cybersecurity problem of the CV-based TSC systems. 

Connected vehicle (CV) technologies enable data exchange between vehicles and transportation infrastructure. In a CV environment, traffic signal control systems receive CV trajectory data through vehicle-to-infrastructure (V2I) communications to make control decisions. Comparing with existing data collection methods (e.g., from loop-detectors), the CV trajectory data provide much richer information, and therefore have great potentials to improve the system performance by reducing total vehicle delay at signalized intersections. However, this connectivity might also bring cyber security concerns. In this paper, we aim to investigate the security problem of CV-based traffic signal control (CV-TSC) systems. Specifically, we focus on evaluating the impact of falsified data attacks on the system performance. A black-box attack scenario, in which the control logic of a CV-TSC system is unavailable to attackers, is considered. A two-step attack model is constructed. In the first step, the attacker tries to learn the control logic using a surrogate model. Based on the surrogate model, in the second step, the attacker launches falsified data attacks to influence the control systems to make sub-optimal control decisions. In the case study, we apply the attack model to an existing CV-TSC system (i.e., I-SIG) and find intersection delay can be significantly increased. Finally, we discuss some promising defense directions. 

High-performance piezoelectric materials are critical components for electromechanical sensors and actuators. For more than 60 years, the main strategy for obtaining large piezoelectric response has been to construct multiphase boundaries, where nanoscale domains with local structural and polar heterogeneity are formed, by tuning complex chemical compositions. We used a different strategy to emulate such local heterogeneity by forming nanopillar regions in perovskite oxide thin films. We obtained a giant effective piezoelectric coefficient d 33 , f * of ~1098 picometers per volt with a high Curie temperature of ~450°C. Our lead-free composition of sodium-deficient sodium niobate contains only three elements (Na, Nb, and O). The formation of local heterogeneity with nanopillars in the perovskite structure could be the basis for a general approach to designing and optimizing various functional materials. 

A novel and efficient method is demonstrated to improve the electrochemical performance of Li 4 Ti 5 O 12 and metal-oxide anodes. In contrast to other methods, inexpensive red phosphorus powder is used as a reducing reagent, and the reduction is conducted at a relatively low temperature of 400 °C. This method offers a low cost and effective way for Li 4 Ti 5 O 12 and metal-oxide anode applications.